Year: 2015 | Month: June | Volume 8 | Issue 2
Effect of Gamma Irradiation on Vegetative and Propagule Characters in Gladiolus and Induction of Homeotic Mutants
DOI:10.5958/2230-732X.2015.00049.2
Abstract:
Uniform and healthy corms of eight gladiolus varieties ( Yellow Golden, Nathan Red, White Friendship, American Beauty, Red Majesty, Purple Flora and Algarve) were irradiated with different doses (0, 25, 40, 55 and 70 Gy) of gamma rays from 60Co source and planted under open field condition in Randomized Block Design (RBD) with factorial concept. Each treatment was replicated thrice.Plants treated with higher doses (55 Gy and 70 Gy) showed deleterious effect of ionising radiations although at lowest dose (25 Gy) plants were not affected much. Plant height was reduced after irradiation as compare to untreated plants and was recorded minimum at highest doses. Number of shoots per plant was slightly increased at 25 Gy treatment (1.62) in vM2 as compare to untreated plants (1.42). Significant effect of gamma irradiation on corm and cormel characters was noticed. Maximum number of corms per plant was recorded in plants treated with 25 Gy and minimum at 70 Gy. Among all the varieties Nathan Red had maximum number (1.75, 1.97) and weight (73.29gm, 79.33gm) of corms per plant in vM1 as well as vM2 generation. Number of cormels per plant was also reduced with the increase in gamma rays dose. Maximum number of cormels per plant (227.83) was recorded in the interaction of Algarve variety with 25 Gy gamma ray dose in vM2. Homeotic mutants were isolated from all the varieties at 55 and 70 Gy except American Beauty and Red Majesty variety.
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